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3,4-dihydroxybenzoic Acid From Smilacis Chinae Rhizome Protects Amyloid Beta Protein (25-35)-induced Neurotoxicity in Cultured Rat Cortical Neurons

3,4-dihydroxybenzoic Acid From Smilacis Chinae Rhizome Protects Amyloid Beta Protein (25-35)-induced Neurotoxicity in Cultured Rat Cortical Neurons

Ju Yeon Ban, Soon Ock Cho, So-Young Jeon, KiHwan Bae, Kyung-Sik Song, Yeon Hee Seong

Neurosci Lett. 2007 Jun 13;420(2):184-8.

PMID: 17531386

Abstract:

The neuroprotective effect of 3,4-dihydroxybenzoic acid (3,4-DHBA) isolated from Smilacis chinae rhizome against Abeta (25-35)-induced neurotoxicity on cultured rat cortical neurons was found in this study. The protective effect of 3,4-DHBA against Abeta (25-35)-induced neuronal cell death was investigated by measuring cell viability via a 3-[4,5-dimethylthiazol-2-yl]-2,5-diphenyl-tetrazolium bromide (MTT) assay and Hoechst 33342 staining. 3,4-DHBA (1 and 10 microM) concentration-dependently inhibited 10 microM Abeta (25-35)-induced neuronal apoptotic death. 3,4-DHBA (1 and 10 microM) inhibited 10 microM Abeta (25-35)-induced elevation of cytosolic Ca(2+) concentration ([Ca(2+)](c)), which was measured by a fluorescent dye, Fluo-4 AM. 3,4-DHBA also inhibited glutamate release into medium, reactive oxygen species (ROS) generation, and caspase-3 activation, which were induced by 10 microM Abeta (25-35). These results suggest that 3,4-DHBA prevents Abeta (25-35)-induced neuronal cell damage by interfering with the increase of [Ca(2+)](c), and then by inhibiting glutamate release, generation of ROS and caspase-3 activity.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP99503-A	3,4-Dihydroxybenzoic acid		99-50-3	Price

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