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4,4-Diisothiocyanatostilbene Disulfonic Acid Enhanced 15-Deoxy-Δ 12,14-prostaglandin J 2-Induced Neuronal Apoptosis

4,4-Diisothiocyanatostilbene Disulfonic Acid Enhanced 15-Deoxy-Δ 12,14-prostaglandin J 2-Induced Neuronal Apoptosis

Hiromi Koma, Yasuhiro Yamamoto, Hiroaki Kumagai, Tatsurou Yagami

Biol Pharm Bull. 2019;42(11):1913-1920.

PMID: 31685774

Abstract:

4,4-Diisothiocyanatostilbene disulfonic acid (DIDS), an antagonist of anion channel including voltage-dependent anion channel (VDAC), acts as both neurotoxicant and neuroprotectant, resulting in the controversy. VDAC contributes to neuronal apoptosis and is a candidate target protein of 15-deoxy-Δ12,14-prostaglandin J2 (15d-PGJ2). Caspase-3 is activated during neuronal apoptosis caused by 15d-PGJ2. In the present study, we ascertained whether DIDS was neuroprotective or neurotoxic in the primary culture of rat cortical neurons. Neuronal cell viabilities were primarily evaluated by the 3-(4,5-dimethyl-2-thiazolyl)-2,5-diphenyl-2H tetrazolium bromide (MTT) reduction assay. Plasma membrane integrity and apoptosis were detected by the staining of propidium iodide (PI) and Hoechst33342, respectively. Alternatively, apoptosis was also measured by caspase-3 assay kit. DIDS did not prevent neurons from undergoing the 15d-PGJ2-induced apoptosis. In contrast, DIDS caused neuronal cell death in a concentration-dependent manner by itself, confirming its neurotoxicity. The sublethal application of DIDS did not decrease MTT-reducing activity, increase caspase-3 activity, condense chromatin, allow PI to enter neuron and degenerate neuronal morphology significantly. Interestingly, DIDS enhanced the 15d-PGJ2-induced neuronal apoptosis markedly under the sublethal condition. To our knowledge, this is the first report of synergistic effects of DIDS on the neurotoxicity of 15d-PGJ2.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS793902	4-(2-thiazolyl)aminocarbonylphenylboronic acid			Price

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