Home
Resources
Publications
4-(6,7-Dihydro-5,8-dioxothiazolo[4,5-g]phthalazin-2-yl)benzoic Acid N-hydroxysuccinimide Ester as a Highly Sensitive Chemiluminescence Derivatization Reagent for Amines in Liquid Chromatography

4-(6,7-Dihydro-5,8-dioxothiazolo[4,5-g]phthalazin-2-yl)benzoic Acid N-hydroxysuccinimide Ester as a Highly Sensitive Chemiluminescence Derivatization Reagent for Amines in Liquid Chromatography

H Yoshida, R Nakao, T Matsuo, H Nohta, M Yamaguchi

J Chromatogr A. 2001 Jan 12;907(1-2):39-46.

PMID: 11217046

Abstract:

4-(6,7-Dihydro-5,8-dioxothiazolo[4,5-g]phthalazin-2-yl)benzoic acid N-hydroxysuccinimide ester was synthesized as a highly sensitive and selective chemiluminescence derivatization reagent for primary and secondary amines in liquid chromatography. Methyl-n-octylamine, n-nonylamine and n-decylamine were used as model compounds to optimize the derivatization, separation and chemiluminescence reaction conditions. This reagent reacts selectively with amines in the presence of triethylamine to give the highly chemiluminescent derivatives, which produce chemiluminescence by reaction with hydrogen peroxide in the presence of potassium hexacyanoferrate(III) in an alkaline medium. The chemiluminescent derivatives of the three amines can be separated within 20 min by reversed-phase liquid chromatography with isocratic elution, followed by chemiluminescence detection. The detection limits (signal-to-noise ratio=3) for primary and secondary amines are at sub-fmol levels for a 20-microl injection. Furthermore, this method was applicable to the determination of amantadine in human plasma.

Chemicals Related in the Paper:

Catalog Number	Product Name	CAS Number	Price
AP55750613	Maleimidoacetic acid N-hydroxysuccinimide ester	55750-61-3	Price
AP89889521	Biotinamidohexanoyl-6-aminohexanoic acid N-hydroxysuccinimide ester	89889-52-1	Price
IAR42418203	Fluorescein-5-EX N-hydroxysuccinimide ester		Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: