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4-Aminophenyl Boronic Acid Modified Gold Platforms for Influenza Diagnosis

4-Aminophenyl Boronic Acid Modified Gold Platforms for Influenza Diagnosis

Sibel Emir Diltemiz, Arzu Ersöz, Deniz Hür, Rüstem Keçili, Ridvan Say

Mater Sci Eng C Mater Biol Appl. 2013 Mar 1;33(2):824-30.

PMID: 25427493

Abstract:

As a potential pandemic threat to human health, there has been an urgent need for rapid, sensitive, simpler and less expensive detection method for the highly pathogenic influenza A virus. For this purpose, Quartz Crystal Microbalance (QCM) and Surface Plasmon Resonance (SPR) sensors have been developed for the recognition of hemagglutinin (HA) which is a major protein of influenza A virus. 4-Aminophenyl boronic acid (4-APBA) has been synthesized and used as a new ligand for binding of sialic acid (SA) via boronic acid-sugar interaction. SA has an important role in binding of HA. QCM and SPR sensor surfaces have been modified with thiol groups and then 4-APBA and SA have been immobilized on sensor surfaces, respectively. Sensor surfaces have been screened with AFM and used for the determination of HA from aqueous solution. The selective recognition of the QCM and SPR sensors toward Concanavalin A has been reported in this work. Also, the binding capacity and detection limits of QCM and SPR sensors have been calculated and detection limits were found to be 4.7 × 10(-2) μM, (0.26 μg ml(-1)) and 1.28 × 10(-1) μM, (0.72 μg ml(-1)) in the 95% confidence interval, respectively.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS793877	(4-phenylaminocarbonylphenyl)boronic acid			Price

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