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4-Mercaptophenylboronic Acid Functionalized Graphene Oxide Composites: Preparation, Characterization and Selective Enrichment of Glycopeptides

4-Mercaptophenylboronic Acid Functionalized Graphene Oxide Composites: Preparation, Characterization and Selective Enrichment of Glycopeptides

Bo Jiang, Yanyan Qu, Lihua Zhang, Zhen Liang, Yukui Zhang

Anal Chim Acta. 2016 Mar 17;912:41-8.

PMID: 26920771

Abstract:

Selective enrichment and isolation of glycopeptides from complex biological samples was indispensable for mass spectrometry (MS)-based glycoproteomics, however, it remained a great challenge due to the low abundance of glycoproteins and the ion suppression of non-glycopeptides. In this work, 4-mercaptophenylboronic acid functionalized graphene oxide composites were synthesized via loading gold nanoparticles on polyethylenimine modified graphene oxide surface, followed by 4-mercaptophenylboronic acid immobilization by the formation of Au-S bonding (denoted as GO/PEI/Au/4-MPB composites). The composites showed highly specific and efficient capture of glycopeptides due to their excellent hydrophilicity and abundant boronic acid groups. The composites could selectively capture the glycopeptides from the mixture of glycopeptides and nonglycopeptides, even when the amounts of non-glycopeptides were 100 times more than glycopeptides. Compared with commercial meta-amino phenylboronic acid agarose, the composites showed better selectivity when the sample was decreased to 10 ng. These results clearly verified that the GO/PEI/Au/4-MPB composites might be a promising material for glycoproteomics analysis.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP237429333	4-Mercaptophenylboronic acid		237429-33-3	Price

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