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4-Mercaptophenylboronic Acid-Modified Spirally-Curved Mesoporous Silica Nanofibers Coupled With Ultra Performance Liquid Chromatography-Mass Spectrometry for Determination of Brassinosteroids in Plants

4-Mercaptophenylboronic Acid-Modified Spirally-Curved Mesoporous Silica Nanofibers Coupled With Ultra Performance Liquid Chromatography-Mass Spectrometry for Determination of Brassinosteroids in Plants

Miao Chen, Ranhao Wang, Yuqiu Zhu, Minzhuo Liu, Fawei Zhu, Jianbo Xiao, Xiaoqing Chen

Food Chem. 2018 Oct 15;263:51-58.

PMID: 29784327

Abstract:

Herein, for the first time, thiol-functionalized mesoporous silica (mSiO2-SH) nanofibers with a spirally-curved twisted hexagonal morphology were synthesized via a simple one-pot protocol. 4-Mercaptophenylboronic acids (4-MPBA) were attached onto the mSiO2-SH nanofibers via disulfide bond, serving as boronate affinity sorbent to selectively capture brassinosteroids (BRs) from plant extract. The resulting BRs-MPBA derivatives were easily eluted from the sorbent by cleaving the disulfide bond, which was subsequently subjected to ultra performance liquid chromatography-mass spectrometry (UPLC-MS) analysis. Thus, the in situ extraction/derivatization/desorption method coupled with UPLC-MS was established for the fast, sensitive and selective detection of BRs in plant tissues. Finally, based on the developed method, endogenous BRs were successfully detected in leaf of H. lupulus L., silique of A. thaliana, and panicle of O. sativa L.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP237429333	4-Mercaptophenylboronic acid		237429-33-3	Price

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