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5-BDBD Ameliorates an OVA-induced Allergic Asthma by the Reduction of Th2 Cytokines Production

5-BDBD Ameliorates an OVA-induced Allergic Asthma by the Reduction of Th2 Cytokines Production

Bing Hu, Xiaoqian Feng, Li Wang, Yinli Song, Xiuqin Ni

Iran J Basic Med Sci. 2018 Apr;21(4):364-369.

PMID: 29796218

Abstract:

Objectives:
P2X4R is expressed in immunocyte and lung tissues. It has been a focus in inflammatory responses recently. This study investigated whether blockage of P2X4R attenuates allergic inflammation by modulating T cell response in ovalbumin-sensitized mice.
Materials and methods:
Ovalbumin was used to sensitize and challenge for a mouse model. Intranasal application of 5-BDBD, P2X4R antagonist, were performed 3 hr before each airway allergen challenge. The lung was evaluated for P2X4R by real-time PCR and immunofluorescence. Th1/Th2 cytokines in bronchoalveolar lavage fluid were measured by ELISA. T-bet, Gata-3, and p-p38 MAPK were measured by Western blot or real-time PCR.
Results:
P2X4R was overexpressed in the lung after allergen challenge compared with the control group. Blockage of P2X4R decreased inflammation in the lung, IL-4 expression was reduced as well as IL-5; IFN-γ expression was elevated in BALF in ovalbumin-sensitized mice. Moreover, blockage of P2X4R inhibited ovalbumin-induced increased Gata-3 level and decreased T-bet level.
Conclusion:
These findings suggest that 5-BDBD ameliorates an ovalbumin-induced asthmatic attack by the downregulation of cytokines related to the Th2 cell.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP768404031	5-BDBD		768404-03-1	Price

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