99m Tc-Labeled 5-amino-1,3-bis(ethylamine-( N,N-dimethyl diphosphonic acid)acetamido)benzene

Puja Panwar, Sweta Singh, Nitin Kumar, Harish Rawat, Anil Kumar Mishra, Arvind Chopra

PMID: 20641869

Abstract:

Cancers such as those of the breast, prostate, kidney, and thyroid have a high incidence of metastases, particularly in the bone, which results in bone resorption, pain, hypercalcemia, spinal compression, decreased mobility, and even fractures (1). In addition, osteoporosis (bone resorption), a common condition experienced most frequently by menopausal women as well as aging women and men, often leads to bone fractures in these individuals (2). Although chemo- and radiotherapy are often used to treat bone metastases, none of these treatments control the progression of this disease or result in a better prognosis for the patient. Bisphosponates (BPs) or their nitrogen-containing derivatives (N-BPs) are the most commonly used compounds for the selective targeting and treatment of bone-related ailments observed during cancer metastases or osteoporosis (3, 4) because of their attraction to hydroxyapatite, a major component of bone, and because they alleviate, to some extent, the symptoms and complications arising from these conditions. The chemical structure, characteristics, and pharmacological behavior of BPs has been described by Hirabayashi and Fujisaki (3). Briefly, the parent BP compound contains a characteristic phosphate-carbon-phosphate (P-C-P) bond that is resistant to enzymatic digestion and has no substitution at the central carbon (5); N-BPs, however, have a nitrogen-containing moiety substituted on the carbon atom (6). BPs and N-BPs were shown to inhibit bone-related events by different mechanisms (7), and N-BPs were reported to be 100- to 10,000-fold more potent than BPs (8). BPs and N-BPs are approved by the United States Food and Drug Administration for the treatment of bone diseases such as osteoporosis, Paget's disease of the bone, hypercalcemia, and bone metastases. In addition, these drugs also being evaluated in clinical trials for the treatment and imaging of different bone-related disorders.
Accurate and early noninvasive detection of osteoporosis or bone metastases can assist in the development of a suitable treatment strategy and possibly improve the prognosis for a patient. Radiolabeled BPs, such as methyl diphosphonate (MDP) (9), hydroxymethylene diphosphonate, 1-hydroxyethylene diphosphonate, etc., are often used as imaging agents for the detection of bone remodeling (e.g., during cancer metastases) or repair (e.g., after a fracture) because these compounds tend to accumulate in osteoclasts at active bone sites (10). Although radiolabeled N-BPs have been used for the therapy of bone cancer in animal models (11) and humans (12), no N-BPs have been used for bone imaging. Panwar et al. (13) synthesized a 99mTc-labeled multidentated N-BP, 5-amino-1,3-bis(ethylamine-(N,N-dimethyl phosphonic acid)acetamido)benzene (or 5-aminoisophthalate tetramethylene phosphonic acid (99mTc-IPTMP)) and investigated its biodistribution in mice. The accumulation of 99mTc-IPTMP in select organs of the animals was compared to that of the same organs obtained from animals treated with 99mTc-MDP. Whole-body scintigraphic imaging of rabbits treated with the radiolabeled compound was also performed by the investigators.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP252237391	(3,3,4,4,5,5,6,6,7,7,8,8,9,9,10,10,11,11,12,12,12-Heneicosafluorododecyl)phosphonic acid		252237-39-1	Price