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A Bifunctional Spin Label for Ligand Recognition on Surfaces

A Bifunctional Spin Label for Ligand Recognition on Surfaces

Michael A Hollas, Simon J Webb, Sabine L Flitsch, Alistair J Fielding

Angew Chem Int Ed Engl. 2017 Aug 1;56(32):9449-9453.

PMID: 28570782

Abstract:

In situ monitoring of biomolecular recognition, especially at surfaces, still presents a significant technical challenge. Electron paramagnetic resonance (EPR) of biomolecules spin-labeled with nitroxides can offer uniquely sensitive and selective insights into these processes, but new spin-labeling strategies are needed. The synthesis and study of a bromoacrylaldehyde spin label (BASL), which features two attachment points with orthogonal reactivity is reported. The first examples of mannose and biotin ligands coupled to aqueous carboxy-functionalized gold nanoparticles through a spin label are presented. EPR spectra were obtained for the spin-labeled ligands both free in solution and attached to nanoparticles. The labels were recognized by the mannose-binding lectin, Con A, and the biotin-binding protein avidin-peroxidase. Binding gave quantifiable changes in the EPR spectra from which binding profiles could be obtained that reflect the strength of binding in each case.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42411202	Avidin-Peroxidase			Price

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