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A Color-Code for Glycosaminoglycans Identification by Means of Polyacrylamide Gel Electrophoresis Stained With the Cationic Carbocyanine Dye Stains-all

A Color-Code for Glycosaminoglycans Identification by Means of Polyacrylamide Gel Electrophoresis Stained With the Cationic Carbocyanine Dye Stains-all

João Pedro Souza Andrade, Caroline Pacheco Oliveira, Ana Maria Freire Tovar, Paulo Antonio de Souza Mourão, Eduardo Vilanova

Electrophoresis. 2018 Feb;39(4):666-669.

PMID: 29105785

Abstract:

Cationic dyes such as toluidin blue are commonly employed to visualize glycosaminoglycans (GAGs) on electrophoresis gels; however, the carbocyanine-based dye Stains-all have been increasingly used to stain the non-sulfated hyaluronic acid and other GAGs in submicrogram quantities. In this short communication, we demonstrate that Stains-all is able to stain the most common GAGs on polyacrylamide gels with distinct and contrasting colors in a reproducible manner. We also show that this staining method is useful to identify GAGs present both in mixtures and in submicrogram quantities. Therefore, Stains-all has shown to be useful in identifying GAGs on polyacrylamide gels with basis on their specific colors, at least on screening level.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP7423316	Stains-All		7423-31-6	Price

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