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A colorimetric assay for acetylcholinesterase activity and inhibitor screening based on the thiocholine-induced inhibition of the oxidative power of MnO 2 nanosheets on 3,3',5,5'-tetramethylbenzidine

A colorimetric assay for acetylcholinesterase activity and inhibitor screening based on the thiocholine-induced inhibition of the oxidative power of MnO 2 nanosheets on 3,3',5,5'-tetramethylbenzidine

Yuan Sun, Haonan Tan, Yinhuan Li

Mikrochim Acta. 2018 Sep 5;185(10):446.

PMID: 30187211

Abstract:

The authors describe a colorimetric method for the determination of the activity of acetylcholinesterase (AChE). Manganese dioxide (MnO2) nanosheets directly reacts with 3,3',5,5'-tetramethylbenzidine (TMB) in the absence of hydrogen peroxide (H2O2). This leads to the formation of a blue product (oxTMB) with an absorption peak at 652 nm. If AChE hydrolyzes its substrate acetylthiocholine chloride, thiocholine is formed which blocks the oxidative power of the MnO2 nanosheets. Hence, oxTMB will not be formed. The decreased absorbance is directly related to the AChE activity in the 0.01-1.0 mU·mL-1 range. The detection limit is 0.01 mU·mL-1 and the relative standard deviation is 1.2% (for n = 11 at 0.5 mU·mL-1). The method was also applied to screen for inhibitors of AChE. Graphical abstract Based on the oxidizing properties of manganese dioxide nanosheets (MnO2 nanosheets), we report a colorimetric method for determining acetylcholinesterase activity with the chromogenic substrate 3,3',5,5'-tetramethylbenzidine (TMB).

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP54827177	3,3′,5,5′-Tetramethylbenzidine		54827-17-7	Price
AP6050813	Acetylthiocholine chloride		6050-81-3	Price

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