Home
Resources
Publications
A Competitive Enzyme-Linked Immunosorbent Assay (ELISA) to Detect Retronecine and Monocrotaline in Vitro

A Competitive Enzyme-Linked Immunosorbent Assay (ELISA) to Detect Retronecine and Monocrotaline in Vitro

M A Bober, L A Milco, R B Miller, M Mount, B Wicks, M J Kurth

Toxicon. 1989;27(9):1059-64.

PMID: 2508272

Abstract:

Antibodies to the nonesterified pyrrolizidine nucleus, retronecine (155 mol.wt), were produced in rabbits and detected using an avidin-biotin antibody ELISA. A competitive ELISA for the detection of retronecine and the cyclic diester monocrotaline was also developed using the antiserum produced against the hapten conjugate, retronecine-bovine serum albumin. Retronecine was obtained by hydrolysis of monocrotaline, succinylated and directly coupled to bovine serum albumin or ovalbumin. Antibodies to the pyrrolizidine nucleus, retronecine, can be detected within 5 min after the addition of substrate using the avidin-biotin ELISA. Competitive inhibition of antibodies to retronecine is obtained by the addition of known amounts (0-11.42 micrograms/microliters) of either the homologous antigen, retronecine, or the heterologous antigen, monocrotaline, however, retronecine acts as the better competitor.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP480853	Retronecine		480-85-3	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: