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A Coumarin-Based Fluorescence Resonance Energy Transfer Probe Targeting Matrix metalloproteinase-2 for the Detection of Cervical Cancer

A Coumarin-Based Fluorescence Resonance Energy Transfer Probe Targeting Matrix metalloproteinase-2 for the Detection of Cervical Cancer

Guangjie He, Lu Yang, Xinlai Qian, Jing Li, Zhiqing Yuan, Changzheng Li

Int J Mol Med. 2017 Jun;39(6):1571-1579.

PMID: 28487974

Abstract:

Cervical cancer is one of the most common gynecological malignancies worldwide. At present, the methods used for cervical cancer screening have many disadvantages. Matrix metalloproteinase-2 (MMP-2) is low or absent in normal cervical epithelial cells while overexpressed in cervical intraepithelial neoplasia and cervical cancer, which provides the possibility for the detection of cervical cancer based on MMP-2. The development of a strategy with high sensitivity and specificity for cervical cancer detection would be expected to improve the cure rate. Connecting a fluorophore [7-(diethylamino)-2-oxo-2H-chromene-3-succinimidyl ester] with a quencher {4-[4-(dimethylamino)phenylazo]benzoic acid N-succinimidyl ester} via an MMP-2 substrate peptide GPLGVRGKGG, a new coumarin-based fluorescence resonance energy transfer probe targeting MMP-2 was prepared to examine cervical cancer by cell imaging. The cervical cancer cell lines showed green fluorescence with intensities in the order of CaSki > SiHa > C33A > HeLa, which indicated that the cervical cancer cell lines possessed significantly differential expression levels of MMP-2. Quantitative (real‑time) PCR, RT-PCR, and western blot experiments were consistent with such results. The fluorescence detection limit of the probe for MMP-2 was estimated to be 0.05 ng/ml. Therefore, this MMP-2 probe potentially provides a sensitive and specific visual method for cervical cancer screening, diagnosis and prognostic judgment.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP146998314	4-[4-(Dimethylamino)phenylazo]benzoic acid N-succinimidyl ester		146998-31-4	Price
IAR42418264	7-Methoxy-4-coumarinylacetic acid N-succinimidyl ester			Price

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