Home
Resources
Publications
A Design of Experiments Strategy to Enhance the Recovery of Polyphenolic Compounds From

A Design of Experiments Strategy to Enhance the Recovery of Polyphenolic Compounds From

Mirela L Moldovan, Sonia Iurian, Cristina Puscas, Radu Silaghi-Dumitrescu, Daniela Hanganu, Catalina Bogdan, Laurian Vlase, Ilioara Oniga, Daniela Benedec

Biomolecules. 2019 Sep 25;9(10):529.

PMID: 31557922

Abstract:

The aim of the present study was to establish the best experimental conditions that lead to the extracts richest in polyphenolic compounds obtained from pomace and canes of Vitis vinifera. In this regard, a D-Optimal design of experiments (DoE) method was applied to investigate the extraction process parameters from each of three materials: red pomace (RP), white pomace (WP) and canes (C). The input variables were the extraction temperature and the ethanol ratio and as response, the total polyphenols content (TPC) was determined. A design space was generated for each of the plant materials and the most concentrated polyphenol extracts were obtained using 50% ethanol at a temperature of 80 °C. Further, the phenolic profiles of the concentrated extracts were detected by LC/MS/MS and the results showed that WP extract was richer in polyphenolic compounds, both flavonoid and phenolic acids, followed by the RP and C extracts. The antioxidant assays revealed that WP and RP extracts exhibited a higher antioxidant activity which correlated to the high content of polyphenols. These findings revealed that RP, WP and C, currently considered agricultural wastes from winery, may be valorized as an important source of natural antioxidants.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
CS35524	Total Phenolics - WP			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: