Home
Resources
Publications
A Double Staining Technique Using 5-bromo,4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) and Immunoperoxidase in Whole Drosophila Embryos

A Double Staining Technique Using 5-bromo,4-chloro-3-indolyl-beta-D-galactopyranoside (X-gal) and Immunoperoxidase in Whole Drosophila Embryos

S Kobayashi, M Okada

Biotech Histochem. 1993 Jul;68(4):237-9.

PMID: 8218577

Abstract:

We have developed a double staining method using 5-bromo,4-chloro-3-indolyl-beta-D-galactopyranoside X-gal and immunoperoxidase for whole Drosophila embryos. The dechorionated embryos are fixed in heptane saturated with 4% formaldehyde, then in heptane and 50% methanol. Fixed embryos are devitellinized with a tungsten needle and processed for immunoperoxidase staining immediately prior to peroxidase color development. The embryos are stained with X-gal, then peroxidase staining is resumed. This procedure enables us to observe cells stained with both X-gal and a specific antibody in whole embryos.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP7240906	5-Bromo-4-chloro-3-indolyl β-D-galactopyranoside		7240-90-6	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: