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A Far-Red FRET Fluorescent Probe for Ratiometric Detection of L-Cysteine Based on Carbon Dots and N-acetyl-l-cysteine-capped Gold Nanoparticles

A Far-Red FRET Fluorescent Probe for Ratiometric Detection of L-Cysteine Based on Carbon Dots and N-acetyl-l-cysteine-capped Gold Nanoparticles

Wenjuan Dong, Ruiping Wang, Xiaojuan Gong, Wenting Liang, Chuan Dong

Spectrochim Acta A Mol Biomol Spectrosc. 2019 Apr 15;213:90-96.

PMID: 30684884

Abstract:

A novel far-red fluorescence resonance energy transfer (FRET) fluorescent probe for ratiometric detection of l-cysteine (l-Cys) has been designed. The system was established a FRET assembly by positively charged carbon dots (CDs) and negatively charged N-acetyl-l-cysteine capped gold nanoparticles (NAC-AuNPs). The fluorescence of CDs at 539 nm could be effectively quenched in the presence of NAC-AuNPs owing to FRET process, while the emission of NAC-AuNPs at 630 nm was appeared. Subsequently, the interactions between l-Cys and NAC-AuNPs resulted in the decreased emission intensity of NAC-AuNPs, but the emission intensity of CDs kept almost constant due to the continuous FRET efficiency. The ratio of emission intensities at 539 and 630 nm (I539/I630) exhibited a linear correlation to the l-Cys concentration in the range of 1.0-110 μM with the detection limit of 0.16 μM. Moreover, this far-red ratiometric sensor also revealed excellent selectivity toward l-Cys over other amino acids, which showed very high potential in the practical application for diagnosing of cysteine-related disease.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP375395996	Fluorescent red 630		375395-99-6	Price

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