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A Heparin-Mimicking Polymer Conjugate Stabilizes Basic Fibroblast Growth Factor

A Heparin-Mimicking Polymer Conjugate Stabilizes Basic Fibroblast Growth Factor

Thi H Nguyen, Sung-Hye Kim, Caitlin G Decker, Darice Y Wong, Joseph A Loo, Heather D Maynard

Nat Chem. 2013 Mar;5(3):221-7.

PMID: 23422564

Abstract:

Basic fibroblast growth factor (bFGF) is a protein that plays a crucial role in diverse cellular functions, from wound healing to bone regeneration. However, a major obstacle to the widespread application of bFGF is its inherent instability during storage and delivery. Here, we describe the stabilization of bFGF by covalent conjugation with a heparin-mimicking polymer, a copolymer consisting of styrene sulfonate units and methyl methacrylate units bearing poly(ethylene glycol) side chains. The bFGF conjugate of this polymer retained bioactivity after synthesis and was stable to a variety of environmentally and therapeutically relevant stressors--such as heat, mild and harsh acidic conditions, storage and proteolytic degradation--unlike native bFGF. Following the application of stress, the conjugate was also significantly more active than the control conjugate system in which the styrene sulfonate units were omitted from the polymer structure. This research has important implications for the clinical use of bFGF and for the stabilization of heparin-binding growth factors in general.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP106096939-B	Fibroblast Growth Factor-Basic Heparin Stabilized human		106096-93-9	Price

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