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A High-Affinity Protein Stain for Western Blots, Tissue Prints, and Electrophoretic Gels

A High-Affinity Protein Stain for Western Blots, Tissue Prints, and Electrophoretic Gels

D Bickar, P D Reid

Anal Biochem. 1992 May 15;203(1):109-15.

PMID: 1524205

Abstract:

A method for protein staining using copper phthalocyanine 3,4',4'',4'''-tetrasulfonic acid tetrasodium salt is described. The procedure is applicable to protein blots and tissue prints, as well as to polyacrylamide and agarose gels. It is also simple, involving only application of the stain and rinsing. For protein blots and tissue prints the staining is rapid, taking less than 1 min to completion, and more sensitive than any previously described dye-based nonspecific protein staining system. The staining is easily reversible, requiring only a change in pH to remove the dye.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP123439805	Copper phthalocyanine-3,4′,4″,4″′-tetrasulfonic acid tetrasodium salt		123439-80-5	Price

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