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A HPLC-MS/MS Method for Determination of 6'''-feruloylspinosin in Rat Plasma and Tissues: Pharmacokinetics and Tissue Distribution Study

A HPLC-MS/MS Method for Determination of 6'''-feruloylspinosin in Rat Plasma and Tissues: Pharmacokinetics and Tissue Distribution Study

Longdong Qiao, Yan Liu, Xiaoyan Chen, Junbo Xie, Yanqing Zhang, Ke Yang, Hongjian Zhou, Yayun Duan, Wei Zheng, Wenlin Xie

J Pharm Biomed Anal. 2016 Mar 20;121:77-83.

PMID: 26780157

Abstract:

A sensitive, reliable and accurate HPLC-MS/MS method was developed and validated for the quantification of 6'''-feruloylspinosin in rat plasma and tissues with puerarin as the internal standard. The separation was performed on a Proshell 120 EC-C18 column (4.6×150 mm, 2.7 μm) with a mobile phase consisting of acetonitrile and 0.1% formic acid (20:80, v/v) at 0.3 mL/min. The quantification was performed by MRM with m/z [M-H](-) 783.3→427.2 for 6'''-feruloylspinosin and m/z [M-H](-) 415.4→295.4 for the internal standard, respectively. The calibration curves covered over a concentration range of 20-2000 ng/mL in plasma and various tissues samples (heart, liver, spleen, lung, kidney, stomach, intestine, muscle, cerebrum and cerebellum) with good linearity (r(2)≥0.9914). Both the intra- and inter-day precisions were less than 14.70%, and the accuracy (RE%) ranged from -5.80% to 4.93%. The extraction recoveries were within 75.21-92.96%, and the matrix effect ranged from 87.21% to 113.44%. Compared with spinosin, 6'''-feruloylspinosin was distributed in rats faster whereas more slowly eliminated from the plasma. 6'''-Feruloylspinosin could be distributed rapidly and widely in various tissues, and transfer across the blood-brain barrier. In addition, both 6'''-feruloylspinosin and spinosin could enhance the expression of GABAAα1, GABAAα5, GABABR1 mRNA in rat hippocampal neurons significantly, indicating the bioactivity mechanism of 6'''-feruloylspinosin was involved in the GABA receptors.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP319480847	6′′′-Feruloylspinosin		319480-84-7	Price

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