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A Human Monoclonal Antibody That Binds Serotype A Botulinum Neurotoxin

A Human Monoclonal Antibody That Binds Serotype A Botulinum Neurotoxin

Sharad P Adekar, R Mark Jones, M D Elias, Fetweh H Al-Saleem, Michael J Root, Lance L Simpson, Scott K Dessain

Hybridoma (Larchmt). 2008 Feb;27(1):11-7.

PMID: 18294071

Abstract:

Monoclonal antibodies have demonstrated significant potential as therapeutics for botulinum neurotoxin exposures. We previously described a hybridoma method for cloning native human antibodies that uses a murine myeloma cell line that ectopically expresses the human telomerase catalytic subunit gene (hTERT) and the murine interleukin-6 gene (mIL-6). Here we describe a heterohybridoma cell line that ectopically expresses mIL-6 and hTERT and has improved stability of hTERT expression. We fused this cell line to human peripheral blood B cells from a subject who had received the botulinum toxoid vaccine, cloning a high-affinity antibody (13A) specific for serotype A botulinum neurotoxin (BoNT/A). The 13A antibody is an affinity-matured, post-germinal center IgG(1) lambda antibody that has partial neutralization activity in vivo. 13A binds an epitope on BoNT/A that overlaps the binding epitope of an IgG antibody previously shown to fully neutralize a lethal dose of BoNT/A in vivo. The 13A antibody may be useful for diagnostic testing or for incorporation into an oligoclonal therapeutic to counteract BoNT/A exposure.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42411668	IgG3, Lambda from murine myeloma			Price

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