0

A Humanized MDCK Cell Line for the Efficient Isolation and Propagation of Human Influenza Viruses

Kosuke Takada, Chiharu Kawakami, Shufang Fan, Shiho Chiba, Gongxun Zhong, Chunyang Gu, Kohei Shimizu, Sara Takasaki, Yuko Sakai-Tagawa, Tiago J S Lopes, Jayeeta Dutta, Zenab Khan, Divya Kriti, Harm van Bakel, etc.

Nat Microbiol. 2019 Aug;4(8):1268-1273.

PMID: 31036910

Abstract:

Here, we developed hCK, a Madin-Darby canine kidney (MDCK) cell line that expresses high levels of human influenza virus receptors and low levels of avian virus receptors. hCK cells supported human A/H3N2 influenza virus isolation and growth much more effectively than conventional MDCK or human virus receptor-overexpressing (AX4) cells. A/H3N2 viruses propagated in hCK cells also maintained higher genetic stability than those propagated in MDCK and AX4 cells.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42415154 Hck human Hck human Price
qrcode