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A Hybrid Fluorous Monolithic Capillary Column With Integrated Nanoelectrospray Ionization Emitter for Determination of Perfluoroalkyl Acids by Nano-Liquid Chromatography-Nanoelectrospray Ionization-Mass spectrometry/mass Spectrometry

Haiyang Zhang, Junjie Ou, Yinmao Wei, Hongwei Wang, Zhongshan Liu, Hanfa Zou

J Chromatogr A. 2016 Apr 1;1440:66-73.

PMID: 26916593

Abstract:

A hybrid fluorous monolithic column was simply prepared via photo-initiated free radical polymerization of an acrylopropyl polyhedral oligomeric silsesquioxane (acryl-POSS) and a perfluorous monomer (2,2,3,3,4,4,5,5,6,6,7,7-dodecafluoroheptyl acrylate) in UV-transparent fused-silica capillaries within 5min. The physical characterization of hybrid fluorous monolith, including scanning electron microscopy (SEM), Fourier transform infrared (FT-IR) spectroscopy, mercury intrusion porosimetry (MIP) and nitrogen adsorption/desorption measurement was performed. Chromatographic performance was also evaluated by capillary liquid chromatography (cLC). Due to the fluorous-fluorous interaction between fluorous monolith and analytes, fluorobenzenes could well be separated, and the column efficiencies reached 86,600-92,500plates/m at the velocity of 0.87mm/s for alkylbenzenes and 51,900-76,000plates/m at the velocity of 1.10mm/s for fluorobenzenes. Meanwhile, an approach to integrate nanoelectrospray ionization (ESI) emitter with hybrid fluorous monolithic column was developed for quantitative determination of perfluoroalkyl acids by nanoHPLC-ESI-MS/MS. The integration design could minimize extracolumn volume, thus excluding undesirable peak broadening and improving separation performance.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP2993853 2,2,3,3,4,4,5,5,6,6,7,7-Dodecafluoroheptyl acrylate 2,2,3,3,4,4,5,5,6,6,7,7-Dodecafluoroheptyl acrylate 2993-85-3 Price
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