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A Hyperbranched Polyethylenimine Functionalized Stationary Phase for Hydrophilic Interaction Liquid Chromatography

A Hyperbranched Polyethylenimine Functionalized Stationary Phase for Hydrophilic Interaction Liquid Chromatography

Yahui Peng, Yanjie Hou, Feifang Zhang, Guobin Shen, Bingcheng Yang

Anal Bioanal Chem. 2016 May;408(13):3633-8.

PMID: 26970747

Abstract:

A hyperbranched stationary phase for hydrophilic interaction liquid chromatography (HILIC) has been prepared by grafting polyethylenimine (PEI) onto silica gel (termed as PEI-Sil). Rich primary, secondary, and tertiary amino groups associated with PEI render its good hydrophility. More importantly, the hyperbranched structure of PEI molecule is greatly helpful in improving interaction with polar analytes. For several kinds of model polar compounds, including organic acids, nucleosides, nucleic acid bases, amino acids, cephalosporins, and non-reducing sugars, PEI-Sil demonstrated excellent separation performance in terms of running stability, reproducibility, and separation efficiency (e.g., plate count ~74,000/m). In addition, PEI-Sil also exhibited much better separation selectivity toward inorganic anions when operated in the mode of ion chromatography relative to a commercial amino propyl-bonded column.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LPLC81302	Polyethylenimine on silica gel			Price

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