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A Low-Cost Digital Microscope With Real-Time Fluorescent Imaging Capability

A Low-Cost Digital Microscope With Real-Time Fluorescent Imaging Capability

Md Mehedi Hasan, Mohammad Wajih Alam, Khan A Wahid, Sayem Miah, Kiven Erique Lukong

PLoS One. 2016 Dec 15;11(12):e0167863.

PMID: 27977709

Abstract:

This paper describes the development of a prototype of a low-cost digital fluorescent microscope built from commercial off-the-shelf (COTS) components. The prototype was tested to detect malignant tumor cells taken from a living organism in a preclinical setting. This experiment was accomplished by using Alexa Fluor 488 conjugate dye attached to the cancer cells. Our prototype utilizes a torch along with an excitation filter as a light source for fluorophore excitation, a dichroic mirror to reflect the excitation and pass the emitted green light from the sample under test and a barrier filter to permit only appropriate wavelength. The system is designed out of a microscope using its optical zooming property and an assembly of exciter filter, dichroic mirror and transmitter filter. The microscope is connected to a computer or laptop through universal serial bus (USB) that allows real-time transmission of captured florescence images; this also offers real-time control of the microscope. The designed system has comparable features of high-end commercial fluorescent microscopes while reducing cost, power, weight and size.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR424664	Ovalbumin-Atto 488 conjugate			Price

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