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A Method for Easily Customizable Gradient Gel Electrophoresis

A Method for Easily Customizable Gradient Gel Electrophoresis

Andrew J Miller, Brandon Roman, Eric Norstrom

Anal Biochem. 2016 Sep 15;509:12-14.

PMID: 27393767

Abstract:

Gradient polyacrylamide gel electrophoresis is a powerful tool for the resolution of polypeptides by relative mobility. Here, we present a simplified method for generating polyacrylamide gradient gels for routine analysis without the need for specialized mixing equipment. The method allows for easily customizable gradients which can be optimized for specific polypeptide resolution requirements. Moreover, the method eliminates the possibility of buffer cross contamination in mixing equipment, and the time and resources saved with this method in place of traditional gradient mixing, or the purchase of pre-cast gels, are noteworthy given the frequency with which many labs use gradient gel SDS-PAGE.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR424674	Fluorescein-α-D-galactosamine polyacrylamide			Price

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