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A Monoclonal IgM Smooth Muscle Antibody Reactive With Fibroblast Stress Fibres Produced by Immunization With Treponema Pallidum

A Monoclonal IgM Smooth Muscle Antibody Reactive With Fibroblast Stress Fibres Produced by Immunization With Treponema Pallidum

R A Strugnell, J R Underwood, F M Clarke, J S Pedersen, P J Chalmers, S Faine, B H Toh

Clin Exp Immunol. 1983 Jun;52(3):537-42.

PMID: 6347470

Abstract:

A monoclonal IgM smooth muscle antibody secreted by a hybrid (MMI-1) of mouse plasmacytoma NS-1 with spleen cells from mouse immunized with Treponema pallidum was detected by indirect immunofluorescence tests on frozen tissue sections and on acetone fixed monolayers of rat and human fibroblasts. The antibody did not react with acetone fixed smears of T. pallidum but reacted with smooth muscle fibres and with striations of skeletal and cardiac muscle. In non-muscle cells, the antibody stained liver in a 'polygonal' pattern, thymus with accentuated staining of the thymic medulla, renal glomeruli and the brush border and peritubular fibrils of renal tubules. In fibroblast monolayers, the antibody stained stress fibres in an interrupted pattern. Immunoblotting with muscle proteins and the antibody showed labelling of a 100K molecule. The cellular distribution of the mouse monoclonal antibody is similar to that obtained with anti-actin antibody suggesting that the corresponding antigen may be an actin binding protein.

Chemicals Related in the Paper:

Catalog Number	Product Name	Price
IAR42413883	Anti-Actin antibody , Mouse monoclonal	Price
IAR42413885	Monoclonal Anti-Actin antibody produced in mouse	Price
IAR42413889	Monoclonal Anti-Actin, Smooth Muscle antibody produced in mouse	Price
IAR42413891	Anti-Actin, Cardiac antibody, Mouse monoclonal	Price

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