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A Multivariate Study of the Performance of an Ultrasound-Assisted Madder Dyes Extraction and Characterization by Liquid Chromatography-Photodiode Array Detection

A Multivariate Study of the Performance of an Ultrasound-Assisted Madder Dyes Extraction and Characterization by Liquid Chromatography-Photodiode Array Detection

Guillaume Cuoco, Carole Mathe, Paul Archier, Farid Chemat, Cathy Vieillescazes

Ultrason Sonochem. 2009 Jan;16(1):75-82.

PMID: 18617432

Abstract:

An extraction method of madder (Rubia tinctorum) roots dyes is established and optimized to obtain the original chemical composition. A central composite design (CCD) was developed to specify the importance of the three major factors studied (time, temperature and solvent composition) affecting the ultrasound-assisted extraction of this matrix. A preliminary granulometric study of madder roots is realized in the aim to determine the optimal particles size corresponding to the best ultrasound effects. A comparison with the classical extraction method of madder dyes by reflux is described. The identification of the constituents of R. tinctorum is carried out by liquid chromatography coupled with a photodiode array detector (LC-PDA). Anthraquinonic aglycone and heterosidic dyes compounds are characterized by retention time and UV spectrum: alizarin (1,2-dihydroxyanthraquinone), purpurin (1,2,4-trihydroxyanthraquinone), lucidin (1,3-dihydroxy-2-hydroxymethylanthraquinone), rubiadin (1,3-dihydroxy-2-methylanthraquinone), xanthopurpurin (1,3-dihydroxyanthraquinone), pseudopurpurin (1,2,4-trihydroxy-3-carboxyanthraquinone), lucidin primeveroside, ruberythric acid (alizarin primeveroside), galiosin (pseudopurpurin primeveroside) and rubiadin primeveroside. The optimal experimental conditions are 18min, 36 degrees C and 37/63 MeOH/H(2)O (v/v).

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP152841	Ruberythric acid		152-84-1	Price

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