Home
Resources
Publications
A Narrow Open Tubular Column for High Efficiency Liquid Chromatographic Separation

A Narrow Open Tubular Column for High Efficiency Liquid Chromatographic Separation

Huang Chen, Yu Yang, Zhenzhen Qiao, Piliang Xiang, Jiangtao Ren, Yunzhu Meng, Kaiqi Zhang, Joann Juan Lu, Shaorong Liu

Analyst. 2018 Apr 30;143(9):2008-2011.

PMID: 29632901

Abstract:

We report a great feature of open tubular liquid chromatography when it is run using an extremely narrow (e.g., 2 μm inner diameter) open tubular column: more than 10 million plates per meter can be achieved in less than 10 min and under an elution pressure of ca. 20 bar. The column is coated with octadecylsilane and both isocratic and gradient separations are performed. We reveal a focusing effect that may be used to interpret the efficiency enhancement. We also demonstrate the feasibility of using this technique for separating complex peptide samples. This high-resolution and fast separation technique is promising and can lead to a powerful tool for trace sample analysis.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP18623115	Octadecylsilane		18623-11-5	Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: