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A New Isolation and Purification Method for Staphylococcal Protein A Using Membrane Encapsulated Rabbit IgG-agarose

A New Isolation and Purification Method for Staphylococcal Protein A Using Membrane Encapsulated Rabbit IgG-agarose

A Sakoda, H Y Wang

Biotechnol Bioeng. 1989 Oct 20;34(8):1098-103.

PMID: 18588204

Abstract:

A new isolation and purification method for bioproducts using membrane-encapsulated affinity adsorbents was investigated. The new method involves encapsulation of affinity adsorbents, batch adsorption of the bioproduct from whole fermentation broth and rapid batch desorption after dissolution of the capsule membranes. Recovery of protein A from Staphylococcus aureus was used as the model experimental system. Affinity adsorbents such as rabbit IgG-agarose were successfully encapsulated within calcium alginate membranes and used directly to recover protein A from whole cell homogenate containing a number of macromolecular contaminants as well as suspended solids. Both high yield and high purity of protein A were recovered by this method in comparison with various previously reported methods.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42411624	Rabbit IgG−Agarose			Price

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