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A New Method for the Purification of Human Motile Spermatozoa Applying Density-Gradient Centrifugation: Polysucrose Media Compared to Percoll Media

A New Method for the Purification of Human Motile Spermatozoa Applying Density-Gradient Centrifugation: Polysucrose Media Compared to Percoll Media

C Y Andersen, J Grinsted

J Assist Reprod Genet. 1997 Nov;14(10):624-8.

PMID: 9447466

Abstract:

Purpose:
A newly developed method for the isolation of human motile spermatozoa using density-gradient centrifugation was compared with the traditionally used Percoll technique.
Method:
Sperm samples were divided into two equal aliquots, which were purified with either the traditionally performed Percoll technique or a new alternative based on polysucrose/Optiprep media. For each sample the isolation was performed during the same run of the centrifuge.
Results:
The average recovery of progressively motile spermatozoa with the polysucrose/Optiprep method was significantly higher (48 +/- 7%) than with the Percoll method (38 +/- 6%) (n = 18). The average percentage of motile spermatozoa and the motility score were similar in the purified preparations.
Conclusions:
The new polysucrose/Optiprep-based density-gradient centrifugation technique for the isolation of motile human spermatozoa is as good as the traditionally used Percoll method and may replace it in connection with assisted reproduction techniques.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
LS741334	Polysucrose			Price

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