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A Nonradioactive Iodide Uptake Assay for Sodium Iodide Symporter Function

A Nonradioactive Iodide Uptake Assay for Sodium Iodide Symporter Function

Fanny Waltz, Lucie Pillette, Yves Ambroise

Anal Biochem. 2010 Jan 1;396(1):91-5.

PMID: 19733144

Abstract:

The standard assay for sodium iodide symporter (NIS) function is based on the measurement of radioiodide uptake ((125)I) in NIS-expressing cells. However, cost and safety issues have limited the method from being used widely. Here we describe a simple spectrophotometric assay for the determination of iodide accumulation in rat thyroid-derived cells (FRTL5) based on the catalytic effect of iodide on the reduction of yellow cerium(IV) to colorless cerium(III) in the presence of arsenious acid (Sandell-Kolthoff reaction). The assay is fast and highly reproducible with a Z' factor of 0.70. This procedure allows the screening of more than 800 samples per day and can easily be adapted to robotic systems for high-throughput screening of NIS function modulators. Using this method, the potency of several known inhibitors of NIS function was evaluated in a single day with high accuracy and reliability. Measured IC(50) values were essentially identical to those determined using Na(125)I.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP7790876	Cerium(III) iodide		7790-87-6	Price

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