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A Novel Acetylcholinesterase Biosensor Based on Carboxylic Graphene Coated With Silver Nanoparticles for Pesticide Detection

A Novel Acetylcholinesterase Biosensor Based on Carboxylic Graphene Coated With Silver Nanoparticles for Pesticide Detection

Yongjun Liu, Guangcan Wang, Canpeng Li, Qing Zhou, Min Wang, Long Yang

Mater Sci Eng C Mater Biol Appl. 2014 Feb 1;35:253-8.

PMID: 24411376

Abstract:

A novel acetylcholinesterase (AChE) biosensor based on Ag NPs, carboxylic graphene (CGR) and Nafion (NF) hybrid modified glass carbon electrode (GCE) has been successfully developed. Ag NPs-CGR-NF possessed predominant conductivity, catalysis and biocompatibility and provided a hydrophilic surface for AChE adhesion. Chitosan (CS) was used to immobilize AChE on the surface of Ag NPs-CGR-NF/GCE to keep the AChE activities. The AChE biosensor showed favorable affinity to acetylthiocholine chloride (ATCl) and could catalyze the hydrolysis of ATCl with an apparent Michaelis-Menten constant value of 133 μM, which was then oxidized to produce a detectable and fast response. Under optimum conditions, the biosensor detected chlorpyrifos and carbaryl at concentrations ranging from 1.0×10(-13) to 1×10(-8) M and from 1.0×10(-12) to 1×10(-8) M. The detection limits for chlorpyrifos and carbaryl were 5.3×10(-14) M and 5.45×10(-13) M, respectively. The developed biosensor exhibited good sensitivity, stability, reproducibility and low cost, thus providing a promising tool for analysis of enzyme inhibitors. This study could provide a simple and effective immobilization platform for meeting the demand of the effective immobilization enzyme on the electrode surface.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AS25303	Demand - Constant Value			Price

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