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A Novel Ethanol-Tolerant Laccase, Tvlac, From Trametes Versicolor

A Novel Ethanol-Tolerant Laccase, Tvlac, From Trametes Versicolor

Lei Chen, Xiaoming Yi, Fajun Deng, Wei Fang, Xuecheng Zhang, Xiaotang Wang, Zemin Fang, Yazhong Xiao

Biotechnol Lett. 2016 Mar;38(3):471-6.

PMID: 26553026

Abstract:

Objectives:
To produce and characterize novel laccases with ethanol tolerance from Trametes versicolor using agriculture by-products as energy source.
Results:
Trametes versicolor 1017 produces two laccase isoenzymes with a total activity of 10 U ml(-1) within 8 days when using wheat bran and peanut powder as energy sources in liquid culture medium. A novel isoenzyme, named Tvlac, was identified, purified and characterized. Its optimum pH and temperature were from 4.5 to 5 and 55 to 60 °C, respectively. Its activity was stimulated by ethanol at 10 % (v/v) which increased the V 0.
Conclusions:
The biochemical properties of Tvlac substantiate the potential of this enzyme for applications under an aqueous ethanol mixture environment.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP80498153-B	Laccase from Trametes versicolor		80498-15-3	Price

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