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A Novel Flow-Injection Analysis System for Evaluation of Antioxidants by Using Sodium Dichloroisocyanurate as a Source of Hypochlorite Anion

A Novel Flow-Injection Analysis System for Evaluation of Antioxidants by Using Sodium Dichloroisocyanurate as a Source of Hypochlorite Anion

H Ichiba, K Hanami, K Yagasaki, M Tanaka, H Ito, T Fukushima

Drug Discov Ther. 2012 Feb;6(1):44-8.

PMID: 22460428

Abstract:

A flow injection analysis (FIA) system for evaluation of the antioxidant activity of a compound capable of scavenging a hypochlorite anion (OCl⁻), one of the reactive oxygen species (ROS), was developed. Aminophenyl fluorescein (APF), a fluorescence indicator of ROS, was mixed manually with the test compounds and the mixed solution was injected into the FIA system. The injected solution was reacted in-line with OCl⁻, that was produced by using sodium dichloroisocyanurate in the presence of 0.1 M CH3CO2Na in H2O. The fluorescence intensity of fluorescein generated from non-fluorescent APF was significantly attenuated in compounds that had a scavenging effect on OCl⁻. The precision obtained by the FIA system was satisfactory (relative standard deviation < 5.0%) and a rapid assay within 0.5 min per sample was achieved. The proposed FIA system was used to demonstrate that reduced glutathione, dithiothreitol, and 3-methyl-1-phenyl-5-pyrazolone (edaravone) showed a significant scavenging effect on OCl⁻. Therefore, the proposed FIA system can be used as a screening assay for OCl⁻-scavenging compounds.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42414865	Aminophenyl fluorescein solution			Price

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