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A Potentiometric Immunosensor for Enterovirus 71 Based on bis-MPA-COOH Dendrimer-Doped AgCl Nanospheres With a Silver Ion-Selective Electrode

A Potentiometric Immunosensor for Enterovirus 71 Based on bis-MPA-COOH Dendrimer-Doped AgCl Nanospheres With a Silver Ion-Selective Electrode

Ai-Li Sun

Analyst. 2018 Jan 15;143(2):487-492.

PMID: 29227478

Abstract:

Herein a new potentiometric immunoassay for the point-of-care detection of enterovirus 71 (EV71) was developed by using a silver (Ag+) ion-selective electrode (ISE). Initially, the carboxylated dendrimer-doped AgCl nanospheres were synthesized by the reverse micelle method. Then the synthesized nanospheres were used to label a polyclonal mouse anti-EV71 antibody via a typical carbodiimide coupling method. The immunoreaction was executed on a monoclonal anti-EV71 antibody-coated microplate by using biofunctional AgCl nanospheres as the detection antibody. With a sandwich-type immunoassay format, the carried AgCl nanospheres could be dissolved in the presence of NH3·H2O, and the released silver ions were determined with an external silver ion-selective electrode. Under optimal conditions, the shift in the potential increased with the increase in the EV71 concentration, in a wide linear range of 0.3-300 ng mL-1, with a detection limit of 0.058 ng mL-1. Intra- and inter-assay relative standard deviations with identical batches were less than 4.15% and 6.15%, respectively. By validating the spiked serum samples, our system shows consistency with the enzyme-linked immunosorbent assay (ELISA) kit.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP1076207130	bis-MPA-COOH dendrimer		1076207-13-0	Price

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