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A Rapid Microtiter Plate Assay for Measuring the Effect of Compounds on Staphylococcus Aureus Membrane Potential

Daniel R Gentry, Imogen Wilding, John M Johnson, Dongzhao Chen, Katja Remlinger, Cindy Richards, Susan Neill, Magdalena Zalacain, Stephen F Rittenhouse, Michael N Gwynn

J Microbiol Methods. 2010 Nov;83(2):254-6.

PMID: 20801170

Abstract:

We developed a homogenous microtiter based assay using the cationic dye 3, 3'-Diethyloxacarbocyanine iodide, DiOC2(3), to measure the effect of compounds on membrane potential in Staphylococcus aureus. In a screen of 372 compounds from a synthetic compound collection with anti-Escherichia coli activity due to unknown modes of action at least 17% demonstrated potent membrane activity, enabling rapid discrimination of nuisance compounds.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP905964 3,3′-Diethyloxacarbocyanine iodide 3,3′-Diethyloxacarbocyanine iodide 905-96-4 Price
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