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A Rapid Test for Chitinase Activity That Uses 4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide

A Rapid Test for Chitinase Activity That Uses 4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide

M O'Brien, R R Colwell

Appl Environ Microbiol. 1987 Jul;53(7):1718-20.

PMID: 3662513

Abstract:

A total of 101 strains of bacteria from environmental and clinical sources, most of which were gram negative, were tested for chitobiase activity by using a filter paper spot test with 4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide as the substrate. The results were compared with those obtained by a conventional plate method for chitinase activity by using colloidal chitin as the substrate. There was excellent agreement in the results for both methods. The filter paper spot test with 4-methylumbelliferyl-N-acetyl-beta-D-glucosaminide has the advantages of being rapid, simple to perform, and inexpensive. This method should be adaptable to a wider range of microorganisms, particularly those with unusual growth requirements.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP37067304-A	4-Methylumbelliferyl N-acetyl-β-D-glucosaminide		37067-30-4	Price

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