Home
Resources
Publications
A Rat Monoclonal Antibody 4D7 Produced by a Hybridoma Established From a Yolk-Sac Tumor-Bearing Rat Binds Selectively to the Stage-Specific Embryonic Antigen SSEA-1

A Rat Monoclonal Antibody 4D7 Produced by a Hybridoma Established From a Yolk-Sac Tumor-Bearing Rat Binds Selectively to the Stage-Specific Embryonic Antigen SSEA-1

M L Lindvall, T Brodin, H O Sjögren

Int J Cancer. 1987 Jul 15;40(1):94-8.

PMID: 2885279

Abstract:

A monoclonal rat IgM (kappa) antibody (MAb) is produced by a hybridoma obtained by fusion of the rat myeloma Y3 Ag 1.2.3 with spleen cells from a female W/Fu rat bearing a yolk-sac carcinoma isograft. In the rat this antibody (4D7) shows strong selective binding to all tested yolk-sac carcinomas, but no binding to cultured cells of a number of other tumor types or to cells of normal tissues. Immunohistochemical analysis of the specificity of the antibody confirmed the strong binding to yolk-sac carcinomas and also revealed binding to some other rat tissues. These include one colon carcinoma, the embryonic ectoderm and the primitive visceral endoderm of 8.5-day-old embryos, the central nervous system and the oviduct epithelium and some cells in the seminal tubules, the gastrointestinal epithelium and associated mucus and also the distal tubuli and collecting ducts of the kidney. The MAb binds strongly to purified SSEA-1 but not to purified Lewis A glycolipid. It is concluded that the 4D7 MAb recognizes a determinant which is identical to or includes Gal beta 1-4(Fuc alpha 1-3) GlcNAc. The immunogenicity of the SSEA-1 determinant is further confirmed by the demonstration that antibodies binding to purified SSEA-1 but not to Lewis A appear in the sera of some of the rats developing primary yolk-sac carcinoma.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42414505	Monoclonal Anti-CMKLR1 antibody produced in rat			Price
IAR4246036	Monoclonal Anti-PAX9 antibody produced in rat			Price

As a specialist in analytical chemical Products, Alfa Chemistry offers consumers a wealth of raw materials and a full range of technologies and services.

QUICK LINKS

HEADQUARTERS

Tel:

Fax:

Email: