0

A Red-Emitting Naphthofluorescein-Based Fluorescent Probe for Selective Detection of Hydrogen Peroxide in Living Cells

Aaron E Albers, Bryan C Dickinson, Evan W Miller, Christopher J Chang

Bioorg Med Chem Lett. 2008 Nov 15;18(22):5948-50.

PMID: 18762422

Abstract:

We report the synthesis, properties, and cellular application of Naphtho-Peroxyfluor-1 (NPF1), a new fluorescent indicator for hydrogen peroxide based on a red-emitting naphthofluorescein platform. Owing to its boronate cages, NPF1 features high selectivity for hydrogen peroxide over a panel of biologically relevant reactive oxygen species (ROS), including superoxide and nitric oxide, as well as excitation and emission profiles in the far-red region of the visible spectrum (>600nm). Flow cytometry experiments in RAW264.7 macrophages establish that NPF1 can report changes in peroxide levels in living cells.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP61419021 Naphthofluorescein Naphthofluorescein 61419-02-1 Price
qrcode