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A Semiautomated Microfluidic Platform for Real-Time Investigation of Nanoparticles' Cellular Uptake and Cancer Cells' Tracking

A Semiautomated Microfluidic Platform for Real-Time Investigation of Nanoparticles' Cellular Uptake and Cancer Cells' Tracking

Mariana R Carvalho, F Raquel Maia, Joana Silva-Correia, Bruno M Costa, Rui L Reis, Joaquim M Oliveira

Nanomedicine (Lond). 2017 Mar;12(6):581-596.

PMID: 28186438

Abstract:

Aim:
Develop a platform composed of labeled dendrimer nanoparticles (NPs) and a microfluidic device for real-time monitoring of cancer cells fate.
Materials & methods:
Carboxymethylchitosan/poly(amidoamine) dendrimer NPs were labeled with fluorescein-5(6)-isothiocyanate and characterized using different physicochemical techniques. After, HeLa, HCT-116 and U87MG were cultured in the presence of NPs, and cell viability and internalization efficiency in static (standard culture) and dynamic (microfluidic culture) conditions were investigated.
Results:
Cancer cells cultured with NPs in dynamic conditions were viable and presented higher internalization levels as compared with static 2D cultures.
Conclusion:
This work demonstrated that the proposed microfluidic-based platform allows real-time monitoring, which upon more studies, namely, the assessment of an anticancer drug release effect could be used for cancer theranostics.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP27072453	Fluorescein 5(6)-isothiocyanate		27072-45-3	Price

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