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A Sensitive Method of Detecting Proteins on Dot and Western Blots Using a Monoclonal Antibody to FITC

A Sensitive Method of Detecting Proteins on Dot and Western Blots Using a Monoclonal Antibody to FITC

D Samuel, R J Patt, R A Abuknesha

J Immunol Methods. 1988 Mar 16;107(2):217-24.

PMID: 3126242

Abstract:

Monoclonal antibodies to FITC were produced and shown to be specific for the fluorochrome. Molecular weight marker proteins labelled with FITC could be detected after SDS-PAGE and transfer onto nitrocellulose using anti-FITC followed by an anti-mouse IgG-alkaline phosphatase conjugate. The molecular weight of an antigen common to Legionella pneumophila and recognised by a monoclonal antibody could be determined accurately on a Western blot when FITC labelled markers were used as internal standards. The FITC-anti-FITC system was shown to be extremely sensitive, detecting 23.7 amol of BSA-FITC conjugate (equivalent to 1.42 x 10(7) molecules of FITC) in a dot blot assay.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
IAR42427	Monoclonal Anti-FITC−Alkaline Phosphatase antibody produced in mouse			Price

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