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A Simple and Efficient Method for the Extraction and Separation of Menaquinone Homologs From Wet Biomass of Flavobacterium

A Simple and Efficient Method for the Extraction and Separation of Menaquinone Homologs From Wet Biomass of Flavobacterium

Hongfei Wei, Genhai Zhao, Hui Liu, Han Wang, Wenfeng Ni, Peng Wang, Zhiming Zheng

Bioprocess Biosyst Eng. 2018 Jan;41(1):107-113.

PMID: 29075890

Abstract:

Menaquinone homologs (MK-n), that is, MK-4, MK-5, and MK-6, can be produced by the fermentation of Flavobacterium. In this study, we proposed a simple and efficient method for the extraction of menaquinones from wet cells without the process of drying the biomass. Meanwhile, a rapid and effective solution for the separation of menaquinone homologs was developed using a single organic solvent, which was conducive to the recovery of the solvent. The results showed that the highest yield was obtained with pretreatment using absolute ethanol at a ratio of 6:1 (v/m) for 30 min and then two extractions of 30 min each using methanol at a ratio of 6:1 (v/m). The recovery efficiency of the menaquinones reached to 102.8% compared to the positive control. The menaquinone homologs were effectively separated using methanol as eluent at a flow rate of 0.52 mL/min by a glass reverse-phase C18 silica gel column with a height-to-diameter ratio of 5.5:1. The recovery of menaquinones achieved was 99.6%. In conclusion, the methods for extraction and separation of menaquinone homologs from wet Flavobacterium cells were simple and efficient, which makes them suitable not only on a laboratory scale but also for application on a large scale.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP863616-A	Vitamin K2 (MK-4) solution		863-61-6	Price

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