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A Simple and Rapid HPLC Method for the Determination of Atorvastatin in Human Plasma With UV Detection and Its Application to Pharmacokinetic Studies

A Simple and Rapid HPLC Method for the Determination of Atorvastatin in Human Plasma With UV Detection and Its Application to Pharmacokinetic Studies

Afshin Zarghi, Alireza Shafaati, Seyyed Mohsen Foroutan, Arash Khoddam

Arzneimittelforschung. 2005;55(8):451-4.

PMID: 16149712

Abstract:

A rapid and sensitive high-performance liquid chromatographic method for the determination of atorvastatin (CAS 134523-00-5) in plasma was developed in this study. Atorvastatin was isolated from plasma using protein precipitation by acetonitrile. Diltiazem (CAS 33286-22-5) was used as internal standard. The chromatographic conditions were as follows: analytical 125 x 4 mm (i.d.) Nucleosil C8 column (5 microm particle size), mobile phase consisting of sodium dihydrogen phosphate buffer-acetonitrile (60:40, v/v) adjusted to pH 5.5 at a flow rate of 1.5 ml/min, UV detection at 245 nm. The detection limit for atorvastatin in plasma was 1 ng ml(-1). The calibration curve was linear over the concentration range 20-800 ng ml(-1). The recovery was complete. The inter-day and intra-day assay coefficients of variation were found to be less than 7%. The present validated method was successfully used for pharmacokinetic studies of atorvastatin in human subjects.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP33286225-B	Diltiazem, Hydrochloride - CAS 33286-22-5		33286-22-5	Price

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