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A Simple and Rapid Nuclear Staining Method for Rhizoctonia Solani Kuhn

M Seema, B D Punith, N S Devaki

Biotech Histochem. 2012 Apr;87(3):169-71.

PMID: 21745160

Abstract:

We developed a modified staining technique using acridine orange to stain the nuclei of Rhizoctonia solani. Acridine orange solution was prepared in acetic acid buffer, pH 7.2. Staining for 15 min was critical for observing the nuclei. All of the isolates were found to be multinucleated. The nuclei appeared bright green with light orange background. This method is simple, rapid and reproducible.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
AP65612-B Acridine Orange solution Acridine Orange solution 65-61-2 Price
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