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A Simple Histochemical Method for the Identification of Cytotrophoblasts in Tissue Sections

Carolyn J P Jones, Saxon C Whittle, John D Aplin

Placenta. 2016 Jun;42:84-6.

PMID: 27238717

Abstract:

A simple method for the demonstration of placental cytotrophoblast cells is described, utilising the affinity of the lectin from Bandeiraea simplicifolia-II (BSA-II) for intracellular amylase-sensitive glycogen and a protocol using biotinylated BSA-II followed by an avidin-peroxidase revealing system. In early pregnancy, cytotrophoblast cells in chorionic and anchoring villi are deeply stained and with ongoing differentiation the staining gradually decreases in intensity, suggesting that this lectin can be a useful marker for these cells.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42411202 Avidin-Peroxidase Avidin-Peroxidase Price
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