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[A Simple Method for Quantification of Modified Low-Density Lipoproteins]

[A Simple Method for Quantification of Modified Low-Density Lipoproteins]

B B Shoĭbonov, V Iu Baronets, L F Panchenko, A A Pal'tsyn, A A Kubatiev

Patol Fiziol Eksp Ter. Apr-Jun 2012;(2):77-83.

PMID: 22708414

Abstract:

A simple method for quantification of modified low-density lipoproteins (mLDL) in the blood serum containing 8.9% polyvinylpyrrolidone solution 12600 +/- 2700 has been developed. The results show that 10 min incubation of serum in a buffer containing 8.9% PVP leads to complete aggregation mLDL. Atherogenicity of aggregated mLDLs is experimentally confirmed by two independent tests (mLDLs bind and activate the complement system of a guinea pig (pro-inflammatory effect) and cause platelet hyperaggregation (thrombogenic effect)). The proposed method is simple and involves only two steps: mixing the serum with a solution of PVP and recording the turbidity. The method allows to register the presence of mLDL directly in serum without its prior fractionation.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP9003398-D	Polyvinylpyrrolidone solution		9003-39-8	Price

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