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A Simple Microplate-Based Method for the Determination of α-amylase Activity Using the Glucose Assay Kit (GOD Method)

A Simple Microplate-Based Method for the Determination of α-amylase Activity Using the Glucose Assay Kit (GOD Method)

Rizliya Visvanathan, Chathuni Jayathilake, Ruvini Liyanage

Food Chem. 2016 Nov 15;211:853-9.

PMID: 27283705

Abstract:

For the first time, a reliable, simple, rapid and high-throughput analytical method for the detection and quantification of α-amylase inhibitory activity using the glucose assay kit was developed. The new method facilitates rapid screening of a large number of samples, reduces labor, time and reagents and is also suitable for kinetic studies. This method is based on the reaction of maltose with glucose oxidase (GOD) and the development of a red quinone. The test is done in microtitre plates with a total volume of 260μL and an assay time of 40min including the pre-incubation steps. The new method is tested for linearity, sensitivity, precision, reproducibility and applicability. The new method is also compared with the most commonly used 3,5-dinitrosalicylic acid (DNSA) method for determining α-amylase activity.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP6363537-A	Maltose Monohydrate		6363-53-7	Price

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