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A Simple Two-Step Procedure for the Preparation of the First Component of Human Complement (C1) in Its Native Form

A Simple Two-Step Procedure for the Preparation of the First Component of Human Complement (C1) in Its Native Form

J A Schifferli, G Steiger

J Immunol Methods. 1985 Feb 11;76(2):283-8.

PMID: 3973392

Abstract:

Native human C1 was purified from fresh human serum by affinity chromatography on protein A-bound Sepharose in the presence of 4-nitrophenyl-4-guanidinobenzoate hydrochloride (NPGB) taking advantage of the successive binding of IgG to protein A followed by C1 binding to IgG. After elution the C1 preparation contained IgG as a major contaminant as shown by SDS-PAGE. C1 was further purified by gel filtration. The yield of C1 was 12% and less than 4% of this C1 was activated during purification as assessed by a C4 consumption assay.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP19135172	4-Nitrophenyl 4-guanidinobenzoate hydrochloride		19135-17-2	Price

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