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A Simplified Method for Purification of Annexin V From Human Placenta

G G Poghosyan, V Z Melkonyan, M V Mikaelyan, V K Gasparyan

Prep Biochem Biotechnol. 2003 Aug;33(3):209-15.

PMID: 12916812

Abstract:

A simplified procedure for purification of annexin V from human placenta was developed. At first, the protein was separated from other proteins in membrane bound form in the presence of Ca2+, then was extracted with EDTA and purified by affinity chromatography on PAAG-immobilized phosphatidylserine. The purified protein gave a single band with a molecular weight of 35,000 in SDS-PAGE.

Chemicals Related in the Paper:

Catalog Number Product Name Structure CAS Number Price
IAR42412465 Annexin V from human placenta Annexin V from human placenta Price
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