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A Small Molecule Primes Embryonic Stem Cells for Differentiation

A Small Molecule Primes Embryonic Stem Cells for Differentiation

Shoutian Zhu, Heiko Wurdak, Jian Wang, Costas A Lyssiotis, Eric C Peters, Charles Y Cho, Xu Wu, Peter G Schultz

Cell Stem Cell. 2009 May 8;4(5):416-26.

PMID: 19427291

Abstract:

Embryonic stem cells (ESCs) are an attractive source of cells for disease modeling in vitro and may eventually provide access to cells/tissues for the treatment of many degenerative diseases. However, applications of ESC-derived cell types are largely hindered by the lack of highly efficient methods for lineage-specific differentiation. Using a high-content screen, we have identified a small molecule, named stauprimide, that increases the efficiency of the directed differentiation of mouse and human ESCs in synergy with defined extracellular signaling cues. Affinity-based methods revealed that stauprimide interacts with NME2 and inhibits its nuclear localization. This, in turn, leads to downregulation of c-Myc, a key regulator of the pluripotent state. Thus, our findings identify a chemical tool that primes ESCs for efficient differentiation through a mechanism that affects c-Myc expression, and this study points to an important role for NME2 in ESC self-renewal.

Chemicals Related in the Paper:

Catalog Number	Product Name	Structure	CAS Number	Price
AP154589965-A	Stauprimide		154589-96-5	Price

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